Isolation of a Candida albicans gene, tightly linked to URA3, coding for a putative transcription factor that suppresses a Saccharomyces cerevisiae aft1 mutation

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dc.contributor.authorGómez García, Micaela
dc.contributor.authorO'Connor, José-Enrique
dc.contributor.authorLatorre García, Lorena
dc.contributor.authorIrar Martínez, Sami
dc.contributor.authorHerrero Perpiñán, Enrique
dc.contributor.authorDel Castillo Agudo, Lucas
dc.date.accessioned2015-07-16T07:47:30Z
dc.date.embargoEndDate2025-01-01
dc.date.issued2001
dc.description.abstractA pathogen such as C. albicans needs an ef®cient mechanism of iron uptake in an iron- restricted environment such as is the human body. A ferric-reductase activity regulated by iron and copper, and analogous to that in S. cerevisiae, has been described in C. albicans. We have developed an in-plate protocol for the isolation of clones that complement an aft1 mutation in S. cerevisiae that makes cells dependent on iron for growth. After transformation of S. cerevisiae aft1 with a C. albicans library, we have selected clones that grow in conditions of iron de®ciency and share an identical plasmid, pIRO1, with a 4500 bp insert containing the URA3 gene and an ORF (IRO1) responsible for the suppression of the iron dependency. IRO1 does not show homology with AFT1 or with other sequences in the databases. Northern analysis demonstrates constitutive expression of IRO1. CAI4, a C. albicans strain isolated as Dura3, also has a deletion of the 3k half of IRO1, and displays in YNB medium similar phenotypic characteristics to S. cerevisiae aft1 mutant strains. Therefore, we consider IRO1 as a gene of C. albicans involved in the utilization of iron. However, in extreme conditions of iron deprivation, CAI4 seems to activate alternative mechanisms of iron uptake that allow a better growth than the wild strain SC5314. Analysis of its predicted protein sequence is in agreement with a role of Iro1p as a transcription factor.ca_ES
dc.identifier.doihttps://doi.org/10.1002/1097-0061(20010315)18:4<301
dc.identifier.idgrec004201
dc.identifier.issn0749-503X
dc.identifier.urihttp://hdl.handle.net/10459.1/48601
dc.language.isoengca_ES
dc.publisherJohn Wiley & Sonsca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1002/1097-0061(20010315)18:4<301ca_ES
dc.relation.ispartofYeast, 2001, vol. 18, p. 301-311ca_ES
dc.rights(c) John Wiley & Sons, 2001ca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.subjectCandida albicansca_ES
dc.subjectIron uptakeca_ES
dc.subjectTranscription factorca_ES
dc.titleIsolation of a Candida albicans gene, tightly linked to URA3, coding for a putative transcription factor that suppresses a Saccharomyces cerevisiae aft1 mutationca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
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