Transcription factor OsBZ8 directly activates multiple methylerythritol 4-phosphate and the mevalonate pathway genes in rice seeds

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dc.contributor.authorHuang, Xin
dc.contributor.authorHe, Wenshu
dc.contributor.authorJin, Xin
dc.contributor.authorBassie Rene, Ludovic
dc.contributor.authorVoltas, Jordi
dc.contributor.authorChristou, Paul
dc.contributor.authorCapell Capell, Teresa
dc.date.accessioned2025-07-31T12:16:03Z
dc.date.available2025-07-31T12:16:03Z
dc.date.issued2025
dc.description.abstractThe rice transcription factor OsBZ8 binds to the core sequence ACGT in a G-box (CACGTG), hybrid G/C-box (GACGTG) or abscisic acid response element (ACGTGGC) in the upstream promoter of its target genes. The expression of this transcription factor is largely restricted to seeds 25 days after pollination, coinciding with isoprenoid accumulation, suggesting it may be responsible for the coordinated regulation of the mevalonate (MVA) and the methylerythritol 4-phosphate (MEP) pathway genes. We therefore screened the corresponding promoters for relevant cis-acting elements in 14 genes (three isoforms each of AACT, HMGS, HMGR and DXS and two of IPPI) involved in the MVA/MEP pathways and found a G-box or hybrid G/C-box motif within the first 300 bp upstream of five genes (OsAACT3, OsHMGS1, OsHMGR1, OsDXS2 and OsIPPI1). The expression profile of all five genes in rice seeds matched that of OsBZ8, with stronger expression in the embryo than the endosperm. The specific binding of OsBZ8 to three of the promoter elements was confirmed in yeast one-hybrid assays, and reporter gene constructs provided direct evidence of transactivation in a representative gene. Our findings provide insight into the regulation of isoprenoid metabolism in rice and will facilitate the use of transcription factors in rice seeds to boost the production of target metabolites
dc.description.sponsorshipMinisterio de Ciencia e Innovación,Grant/Award Number: MCIN/AEI/10.13039/501100011033/FEDER;Generalitat de Catalunya, Grant/AwardNumber: 2021 SGR 01501; Next GenerationEU/PRTR, Grant/Award Number:TED2021-129400B-I00
dc.identifier.doihttps://doi.org/10.1111/aab.70036
dc.identifier.idgrec035673
dc.identifier.issn0003-4746
dc.identifier.urihttps://hdl.handle.net/10459.1/468477
dc.language.isoeng
dc.publisherJohn Wiley and Sons Inc
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021‑2023/TED2021‑129400B‑I00/ES/REDUCCIÓN DEL IMPACTO MEDIOAMBIENTAL DE LAS PRÁCTICAS AGRÍCOLAS: DESARROLLO DE PROTOTIPOS DE LÍNEAS DE ARROZ RESISTENTES A LA PIRICULARIA
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1111/aab.70036
dc.relation.ispartofAnnals of Applied Biology, 2025. In press.
dc.rightscc-by-nc-nd (c) Huang et al., 2025
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectbZIP transcription factor
dc.subjectDNA-binding motif
dc.subjectG-box; hybrid G/C-box
dc.titleTranscription factor OsBZ8 directly activates multiple methylerythritol 4-phosphate and the mevalonate pathway genes in rice seeds
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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